This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
3D structures
This page shows the available 3D structures for the domains discussed in Protein Domains page. The figures and functional descriptions were obtained from the cited database, with the corresponding literature citations listed in the reference section.
This page shows the available 3D structures for the domains discussed in Protein Domains page. The figures and functional descriptions were obtained from the cited database, with the corresponding literature citations listed in the reference section.
EGF_CA domain
Function: Calcium-binding; may be important for protein-protein interaction
Database: Pfam
Function: Calcium-binding; may be important for protein-protein interaction
Database: Pfam
NL domain (also termed LNR and DSL in SMART, NOTCH and NODP in Pfam)
Function: Negative regulation of Notch1 activity
Database: SMART, Pfam
Function: Negative regulation of Notch1 activity
Database: SMART, Pfam
Analysis:
All databases used above are very useful in finding the 3D structures for human Notch1 protein, perhaps because the Notch1 protein has been widely studied. However, there are also some uncharacterized domains in which no 3D structures could be defined. As stated in the Protein Domains section, the Notch1 mutations associated with T-ALL seems to fall in the uncharacterized regions. Hence, it may be important to carry out further research in these regions and define its structure and functions.
References:
1. Downing AK, Knott V, Werner JM, Cardy CM, Campbell ID, Handford PA; , Cell 1996;85:597-605.: Solution structure of a pair of calcium-binding epidermal growth factor-like domains: implications for the Marfan syndrome and other genetic disorders. PUBMED:8653794
2. Bork P, Downing AK, Kieffer B, Campbell ID; , Q Rev Biophys 1996;29:119-167.: Structure and distribution of modules in extracellular proteins. PUBMED:8870072
3. Vardar D, North CL, Sanchez-Irizarry C, Aster JC, Blacklow SC; , Biochemistry. 2003;42:7061-7067.: Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1. PUBMED:12795601
4. Boldt HB, Kjaer-Sorensen K, Overgaard MT, Weyer K, Poulsen CB, Sottrup-Jensen L, Conover CA, Giudice LC, Oxvig C; , J Biol Chem. 2004;279:38525-38531.: The Lin12-notch repeats of pregnancy-associated plasma protein-A bind calcium and determine its proteolytic specificity. PUBMED:15262980
5. M.T.Ehebauer et al. (2005). High-resolution crystal structure of the human Notch 1 ankyrin domain. Biochem J, 392, 13-20. PubMed id: 16011479Ref: Full text